Solubilization by lysolecithin and purification of the plasma membrane ATPase of the yeast Schizosaccharomyces pombe.
نویسندگان
چکیده
منابع مشابه
The purified plasma membrane ATPase of the yeast Schizosaccharomyces pombe forms a phosphorylated intermediate.
An acid slab gel electrophoresis method of high-resolving power allows detection of a phosphorylated form in the purified ATPase of the yeast Schizosaccharomyces pombe and identification of this catalytic intermediate among the different phosphopeptides of a plasma membrane preparation. At a maximum steady state rate of MgATP hydrolysis by the membrane-bound ATPase, 20 to 40% of the ATPase subu...
متن کاملSolubilization and purification of the Neurospora plasma membrane H+-ATPase.
The electrogenic proton-translocating ATPase in the plasma membrane of Neurospora has been solubilized with lysolecithin and purified using a combination of gel filtration and density gradient centrifugation. Isolated plasma membrane vesicles are solubilized with lysolecithin in the presence of MgATP, vanadate, and chymostatin. The MgATP and vanadate are required to maintain the ATPase in an ac...
متن کاملExchange of oxygen between phosphate and water catalyzed by the plasma membrane ATPase from the yeast Schizosaccharomyces pombe.
The ATPase of the plasma membrane isolated from the yeast Schizosaccharomyces pombe catalyses a medium Pi in equilibrium H2O exchange in the presence of Mg2+ and in the absence of ATP and ADP. (formula, see text) The Pi in the E.Pi species tumbles in the active site so that each of its oxygens has an equal probability of exchange with water. The partition coefficient (Pc = k2/k2 + k-1) is 0.45....
متن کاملEfflux of potassium induced by dio-9, a plasma membrane ATPase inhibitor in the yeast Schizosaccharomyces pombe.
The active uptake of L-leucine is 50% inhibited by 10 pg of Dio-S/ml in glycolyzing cells of the yeast Schizosaccharomyces pombe in which the respiration has been inhibited by antimycin. Similar inhibition is obsetied in the nuclear respiratory-deficient mutant RD32 which lacks mitochondrial ATPase activity. Therefore the mitochondrial ATPase is not involved in the inhibition by Dio-9 of the ce...
متن کاملstudy of cohesive devices in the textbook of english for the students of apsychology by rastegarpour
this study investigates the cohesive devices used in the textbook of english for the students of psychology. the research questions and hypotheses in the present study are based on what frequency and distribution of grammatical and lexical cohesive devices are. then, to answer the questions all grammatical and lexical cohesive devices in reading comprehension passages from 6 units of 21units th...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1978
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)38024-9